CypHer5E Maleimide

Excitation (nm): 643. Emission (nm): 660 CypHer5E maleimide is an excellent building block for developing site-specific pH probes. It can be readily conjugated to thiol-containing biomolecules (such as antibodies, peptides and thiol-modified oligos). CypHer5E is a red laser-excitable, pH-sensitive cyanine dye derivative that can be used to study most classes of cell surface receptors which internalize into the endocytic pathway when activated by a ligand. CypHer5E maleimide is a flexible, generic fluorophore that can be coupled to a receptor or epitope tag antibody and monitors neutral to acidic intracellular events. CypHer5E has an excitation peak that perfectly matches the red laser at 633 nm of He-Ne laser and 647 nm of red laser and an emission peak at ~670 nm.