Cal Green(TM) 1, AM [Equivalent to Calcium Green -1, AM]

Ex: 506 nm. Em: 531 nm. Soluble in: DMSO. Cal GreenT 1 (AAT Bioquest) is the same molecule to Calcium Green-1 (Invitrogen). It exhibits an increase in fluorescence intensity upon binding calcium ion. The cell-impermeant dye, Cal Green 1 AM (Calcium Green-1 AM) is a 488 nm-excitable calcium indicator. Compared to Fluo-3 AM, Cal Green-1 AM is more fluorescent at low calcium concentrations in cells, facilitating the determination of baseline calcium levels and increasing the visibility of resting cells. It has been used in many calcium signaling investigations, including measuring intracellular calcium, following calcium influx and release, and multiphoton excitation imaging of calcium in living tissues. Cells can be loaded with Cal Green-1 AM by adding the dissolved indicator directly to cultured cells in medium. The fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry.