WRW4 (trifluoroacetate salt)

WRW4 is a peptide antagonist of formyl peptide receptor 2 (FPR2, IC50 = 0.23 µM) and FPR3, previously known as FPR-like 1 (FPRL1) and FPRL2, respectively. It inhibits increases in intracellular calcium and chemotaxis induced by the FPR1 and FPR2 agonist WKYMVm (Cay-33589) in RBL-2H3 cells expressing FPR2 and by the FPR3 agonist F2L in isolated human monocytes when used at a concentration of 10 µM. WRW4 (10 µM) also prevents the production of superoxide induced by amyloid-beta (1-42) (Abeta42, Cay-20574) in isolated human neutrophils. In vivo, WRW4 (1 mg/kg) increases lung edema and bronchoalveolar lavage fluid (BALF) protein levels in the absence and presence of the antibiotic ceftriaxone (Cay-18866) in a mouse model of acute lung injury induced by S. pneumoniae.Formal Name: L-tryptophyl-L-arginyl-L-tryptophyl-L-tryptophyl-L-tryptophyl-L-tryptophanamide, trifluoroacetate salt. Synonyms: Trp-Arg-Trp-Trp-Trp-Trp-CONH2, Trp-Arg-Trp-Trp-Trp-Trp-NH2, WRWWWW-NH2. Molecular Formula: C61H65N15O6 . XCF3COOH. Formula Weight: 1104.3. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 12 mg/ml, DMSO: 10 mg/ml, Ethanol: 20 mg/ml, PBS (pH 7.2): 0.20 mg/ml. SMILES: O=C([C@H](CCCNC(N)=N)NC([C@@H](N)CC1=CNC2=CC=CC=C21)=O)N[C@@H](CC3=CNC4=C3C=CC=C4)C(N[C@@H](CC5=CNC6=C5C=CC=C6)C(N[C@@H](CC7=CNC8=C7C=CC=C8)C(N[C@@H](CC9=CNC%10=C9C=CC=C%10)C(N)=O)=O)=O)=O.OC(C(F)(F)F)=O. InChi Code: InChI=1S/C61H65N15O6.C2HF3O2/c62-44(24-34-29-67-45-17-6-1-12-39(34)45)56(78)72-50(22-11-23-66-61(64)65)57(79)74-53(27-37-32-70-48-20-9-4-15-42(37)48)59(81)76-54(28-38-33-71-49-21-10-5-16-43(38)49)60(82)75-52(26-36-31-69-47-19-8-3-14-41(36)47)58(80)73-51(55(63)77)25-35-30-68-46-18-7-2-13-40(35)46,3-2(4,5)1(6)7/h1-10,12-21,29-33,44,50-54,67-71H,11,22-28,62H2,(H2,63,77)(H,72,78)(H,73,80)(H,74,79)(H,75,82)(H,76,81)(H4,64,65,66),(H,6,7)/t44-,50-,51-,52-,53-,54-,/m0./s1. InChi Key: KIPTTXFGLVJUMS-HNZNYIAHSA-N.