Tide Quencher(TM) 5.1WS succinimidyl ester [TQ5.1WS SE]

Molecular weight: 1137.43. Tide Quencher(TM) 5.1WS (TQ5.1WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy5, Cy5.5, Alexa Fluor(R) 647, Alexa Fluor(R) 647, iFluor(R) 647 and iFluor(R) 680. It is an improved version of TQ5 and QSY 21 and BHQ3). TQ5.1WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency. Tide Quencher(TM) 5.1WS succinimidyl ester is primarily used for the post-labeling of amino-modified oligonucleotides and the N-terminal or lysine residues of peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ5.1WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ5.1WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively 'quenching' the fluorescence signal.