Tide Quencher(TM) 5.1 CPG [TQ5.1 CPG] *1000 Å*

Tide Quencher(TM) 5.1 (TQ5.1) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy5, Cy5.5, Alexa Fluor(R) 647, Alexa Fluor(R) 647, iFluor(R) 647 and iFluor(R) 680. It is an improved version of TQ5 and QSY 21 and BHQ3). TQ5.1 is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency. Tide Quencher(TM) 5.1 CPG is an excellent building block for preparing TQ5.1-labeled oligonucleotides. The oligo prepared from (TQ5.1 CPG) may be deprotected in 0.05M potassium carbonate in methanol for 4 hours at room temperature for 2 hours. Alternatively, the oligo may be deprotected in ammonium hydroxide at room temperature for 24-36 hours. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ5.1WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified.