Tetramethylrhodamine isothiocyanate (mixed isomers)

Tetramethylrhodamine isothiocyanate (TRITC) is an amine-reactive derivative of rhodamine that is used as a fluorescent label for antibodies and other probes (ex/em max = 552/575 nm). It consists of a base tetramethylrhodamine molecule functionalized with an isothiocyanate reactive group at one of two hydrogen atoms on the bottom ring of the structure. TRITC is reactive towards primary amine groups on proteins, peptides, and other biomolecules.Formal Name: 9-(2-carboxyisothiocyanatophenyl)-3,6-bis(dimethylamino)-xanthylium, inner salt. CAS Number: 95197-95-8. Synonyms: 5(6)-Tetramethylrhodamine isothiocyanate, 5(6)-TRITC, TRITC. Molecular Formula: C25H21N3O3S. Formula Weight: 443.5. Purity: >95%. Emission: 575 nm. Excitation: 552 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 5 mg/ml, DMSO: 5 mg/ml, DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml. lambdamax: 215, 256, 283, 356, 545 nm. SMILES: [O-]C(C(C=C(N=C=S)C=C1)=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)=O.[O-]C(C(C(N=C=S)=CC=C5)=C5C6=C7C=CC(N(C)C)=CC7=[O+]C8=C6C=CC(N(C)C)=C8)=O. InChi Code: InChI=1S/2C25H21N3O3S/c1-27(2)16-6-9-19-22(12-16)31-23-13-17(28(3)4)7-10-20(23)24(19)18-8-5-15(26-14-32)11-21(18)25(29)30,1-27(2)15-8-10-17-21(12-15)31-22-13-16(28(3)4)9-11-18(22)23(17)19-6-5-7-20(26-14-32)24(19)25(29)30/h2*5-13H,1-4H3. InChi Key: DTICYSNQBDFZSM-UHFFFAOYSA-N.