TAK-242

TAK-242 is an antagonist of toll-like receptor 4 (TLR4) with an IC50 value of 0.68 µM in a secreted alkaline phosphatase (SEAP) assay. It selectively binds to TLR4 over TLR1-3 and TLR5-10 when used at a concentration of 100 nM. TAK-242 (1 µM) prevents the interaction of TLR4 with its adaptor proteins TIRAP or TRAM in HEK293 cells expressing the human receptors. It inhibits IFN-gamma- and LPS-induced production of nitric oxide (NO), TNF-alpha, IL-6, and IL-1beta in isolated mouse peritoneal macrophages (IC50s = 5.7-11 nM), as well as inhibits LPS-induced NETosis in isolated human neutrophils when used at concentrations ranging from 5 to 20 µM. TAK-242 (0.3, 1, and 3 mg/kg) improves survival in a mouse model of septic shock induced by LPS.Formal Name: 6R-[[(2-chloro-4-fluorophenyl)amino]sulfonyl]-1-cyclohexene-1-carboxylic acid, ethyl ester. CAS Number: 243984-11-4. Synonyms: Resatorvid. Molecular Formula: C15H17NO4ClFS. Formula Weight: 361.8. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 10 mg/ml, DMSO: 10 mg/ml, Ethanol: 20 mg/ml. SMILES: FC1=CC(Cl)=C(NS([C@@H]2CCCC=C2C(OCC)=O)(=O)=O)C=C1. InChi Code: InChI=1S/C15H17ClFNO4S/c1-2-22-15(19)11-5-3-4-6-14(11)23(20,21)18-13-8-7-10(17)9-12(13)16/h5,7-9,14,18H,2-4,6H2,1H3/t14-/m1/s1. InChi Key: LEEIJTHMHDMWLJ-CQSZACIVSA-N.