Spautin-1

Spautin-1 is an autophagy inhibitor, inducing cell death in human breast cancer Bcap-37 cells grown in glucose-free media (EC50 = ~1.25 µM). It does not significantly affect the viability of Bcap-37 cells grown in complete media. Spautin-1 promotes the degradation of Vps34 PI3K complex by inhibiting the deubiquitinating enzymes USP10 and USP13 (IC50s = 0.58 and 0.69 µM, respectively). It is used to elucidate the role of autophagy in cellular processes. Spautin-1 has also been used to demonstrate roles for autophagy in viral infection.Formal Name: 6-fluoro-N-[(4-fluorophenyl)methyl]-4-quinazolinamine. CAS Number: 1262888-28-7. Molecular Formula: C15H11F2N3. Formula Weight: 271.3. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml, DMSO: 15 mg/ml, Ethanol: 10 mg/ml. lambdamax: 225, 283, 323, 336 nm. SMILES: FC1=CC(C(NCC2=CC=C(F)C=C2)=NC=N3)=C3C=C1. InChi Code: InChI=1S/C15H11F2N3/c16-11-3-1-10(2-4-11)8-18-15-13-7-12(17)5-6-14(13)19-9-20-15/h1-7,9H,8H2,(H,18,19,20). InChi Key: AWIVHRPYFSSVOG-UHFFFAOYSA-N.