SMCC

SMCC is a heterobifunctional cross-linking reagent that contains an amine-reactive N-hydroxysuccinimide ester and a sulfhydryl-reactive maleimide group that can be used in the conjugation of proteins. It has been used to cross-link the anticancer agent DM1 (mertansine, Cay-22483) to an IgG1 monoclonal antibody to form the antibody-drug conjugate DM1-SMCC (Cay-23926). Immunization of mice with SMCC-coupled keyhole limpet hemocyanin (KLH) antigen and syngeneic splenocytes increases the percentage of proliferating T cells in isolated splenocytes from recipient mice compared to mice immunized with KLH and the adjuvant LPS.Formal Name: 4-[(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)methyl]-cyclohexanecarboxylic acid, 2,5-dioxo-1-pyrrolidinyl ester. CAS Number: 64987-85-5. Synonyms: NSC 344483, Succinimidyl 4-(N-maleimidomethyl)cyclohexan-1-carboxylate. Molecular Formula: C16H18N2O6. Formula Weight: 334.3. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 15mg/ml, DMF:PBS (pH 7.2) (1:4): 0.2mg/ml, DMSO: 10mg/ml. lambdamax: 217 nm. SMILES: O=C(ON1C(CCC1=O)=O)C(CC2)CCC2CN(C(C=C3)=O)C3=O. InChi Code: InChI=1S/C16H18N2O6/c19-12-5-6-13(20)17(12)9-10-1-3-11(4-2-10)16(23)24-18-14(21)7-8-15(18)22/h5-6,10-11H,1-4,7-9H2. InChi Key: JJAHTWIKCUJRDK-UHFFFAOYSA-N.