SJB2-043

SJB2-043 is an inhibitor of the ubiquitin-specific peptidase 1 (USP1) and USP1-associated factor 1 (UAF1) complex (USP1-UAF1 complex, IC50 = 0.544 µM for its deubiquitinase activity) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) papain-like protease (PLpro). It reduces the levels of USP1, DNA-binding protein inhibitor 1 (ID-1), ID-2, and ID-3 in K562 chronic myelogenous leukemia (CML) cells when used at concentrations ranging from 0.5 to 10 µM, an effect that can be partially blocked by the proteasome inhibitor MG132. SJB2-043 decreases the viability of K562 cells (EC50 = 1.07 µM) and induces apoptosis in K562 cells in a concentration-dependent manner. It also inhibits SARS-CoV-2 PLpro with IC50 values of 0.56 and 0.091 µM using the fluorogenic substrates Z-LRGG-AMC (Cay-26641) or ubiquitin-AMC (Ub-AMC), respectively, as PLpro substrates.Formal Name: 2-phenyl-naphth[2,3-d]oxazole-4,9-dione. CAS Number: 63388-44-3. Molecular Formula: C17H9NO3. Formula Weight: 275.3. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 2 mg/ml, DMSO: 1 mg/ml, Ethanol: insol, PBS (pH 7.2): insol. lambdamax: 277 nm. SMILES: O=C1C2=C(C(C3=C1C=CC=C3)=O)OC(C4=CC=CC=C4)=N2. InChi Code: InChI=1S/C17H9NO3/c19-14-11-8-4-5-9-12(11)15(20)16-13(14)18-17(21-16)10-6-2-1-3-7-10/h1-9H. InChi Key: CMYQQADDUUDCCA-UHFFFAOYSA-N.