(R)-nitro-Blebbistatin

(R)-nitro-Blebbistatin is a more stable form of (+)-blebbistatin (Cay-13165), which is the inactive form of (-)-blebbistatin (Cay-13013). Prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive product via cytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications. The addition of a nitro group stabilizes the molecule to circumvent its degradation by prolonged blue light exposure. (R)-nitro-Blebbistatin has the same stereochemistry as the inactive (+)-blebbistatin enantiomer.Formal Name: (3aR)-1,2,3,3a-tetrahydro-3a-hydroxy-7-nitro-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one. CAS Number: 1217619-62-9. Synonyms: R-(-)-7-Desmethyl-8-nitro Blebbistatin. Molecular Formula: C17H13N3O4. Formula Weight: 323.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 20 mg/ml, DMSO: 25 mg/ml, DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml. lambdamax: 234, 270, 315, 350, 443 nm. SMILES: O=C1[C@]2(O)C(N(C3=CC=CC=C3)CC2)=NC4=C1C=CC([N+]([O-])=O)=C4. InChi Code: InChI=1S/C17H13N3O4/c21-15-13-7-6-12(20(23)24)10-14(13)18-16-17(15,22)8-9-19(16)11-4-2-1-3-5-11/h1-7,10,22H,8-9H2/t17-/m0/s1. InChi Key: VIMYHNYWFMRDKQ-KRWDZBQOSA-N.