NLRP3i

NLRP3i is an intermediate in the synthesis of glyburide (Cay-15009) and an inhibitor of the NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome. It is selective for NLRP3 over NLRP4 and absent in melanoma 2 (AIM2) inflammasomes at 400 µM. NLRPi inhibits LPS-induced IL-1beta release and apoptosis-associated speck-like protein containing a CARD (ASC) oligomerization in J774A.1 mouse macrophages. In vivo, NLRP3i (100 mg/kg) reduces infarct size and cardiac levels of troponin I in a mouse model of acute myocardial infarction. It reduces peritoneal leukocyte infiltration in a mouse model of peritonitis induced by zymosan A (Cay-21175) in a dose-dependent manner. NLRP3i (100 mg/kg) also decreases plasma IL-18 levels and reduces systolic and diastolic dysfunction in a mouse model of Western diet-induced obesity.Formal Name: N-[2-[4-(aminosulfonyl)phenyl]ethyl]-5-chloro-2-methoxy-benzamide. CAS Number: 16673-34-0. Molecular Formula: C16H17ClN2O4S. Formula Weight: 368.8. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, DMSO:PBS (pH 7.2) (1:7): 0.14 mg/ml. lambdamax: 227 nm. SMILES: ClC1=CC=C(OC)C(C(NCCC2=CC=C(S(N)(=O)=O)C=C2)=O)=C1. InChi Code: InChI=1S/C16H17ClN2O4S/c1-23-15-7-4-12(17)10-14(15)16(20)19-9-8-11-2-5-13(6-3-11)24(18,21)22/h2-7,10H,8-9H2,1H3,(H,19,20)(H2,18,21,22). InChi Key: KVWWTCSJLGHLRM-UHFFFAOYSA-N.