NGI 1

NGI 1 is an oligosaccharyltransferase (OST) inhibitor. It binds to and stabilizes the STT3B catalytic subunit of OST in a cellular thermal shift assay (CETSA) and inhibits N-linked glycosylation in a reporter assay using D54 ER-LucT cells (IC50 = 1.1 µM). NGI 1 (10 µM) reduces glycosylation and membrane localization of EGFR in H3255 lung adenocarcinoma cells and inhibits proliferation of PC-9 and A549 non-small cell lung cancer (NSCLC) cells. It inhibits dengue and Zika virus replication in HEK293 cells (EC50s = 0.85 and 2.2 µM, respectively). NGI 1 also reduces viral RNA in HEK293 cells infected with West Nile virus or yellow fever virus.Formal Name: 5-[(dimethylamino)sulfonyl]-N-(5-methyl-2-thiazolyl)-2-(1-pyrrolidinyl)-benzamide. CAS Number: 790702-57-7. Synonyms: N-linked Glycosylation Inhibitor 1. Molecular Formula: C17H22N4O3S2. Formula Weight: 394.5. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 10 mg/ml, DMSO: 10 mg/ml, DMSO:PBS (pH 7.2) (1:3): 0.25 mg/ml. lambdamax: 287 nm. SMILES: O=C(C1=CC(S(N(C)C)(=O)=O)=CC=C1N2CCCC2)NC3=NC=C(C)S3. InChi Code: InChI=1S/C17H22N4O3S2/c1-12-11-18-17(25-12)19-16(22)14-10-13(26(23,24)20(2)3)6-7-15(14)21-8-4-5-9-21/h6-7,10-11H,4-5,8-9H2,1-3H3,(H,18,19,22). InChi Key: QPKGRLIYJGBKJL-UHFFFAOYSA-N.