LDN-0212320

LDN-0212320 is an activator of excitatory amino acid transporter 2 (EAAT2) that increases expression of EAAT2 in PA-EAAT2 cells in a concentration- and time-dependent manner (EC50 = 1.83 µM). It selectively increases EAAT2 protein levels over EAAT1 and EAAT3 protein levels and increases glutamate uptake in primary dissociated neuron and astrocyte mixed cultures when used at concentrations ranging from 1 to 3 µM. LDN-0212320 (3 µM) decreases neuronal loss and degeneration in primary dissociated neuron and astrocyte mixed cultures. In vivo, LDN-0212320 (40 mg/kg, i.p.) increases EAAT2 protein levels and glutamate uptake in mouse forebrain. It also delays motor function decline and increases lifespan in the SOD1G93A transgenic mouse model of amyotrophic lateral sclerosis (ALS).Formal Name: 3-[[(2-methylphenyl)methyl]thio]-6-(2-pyridinyl)-pyridazine. CAS Number: 894002-50-7. Synonyms: OSU-0212320. Molecular Formula: C17H15N3S. Formula Weight: 293.4. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 25 mg/mL, Ethanol: 5 mg/mL. lambdamax: 294 nm. SMILES: CC1=CC=CC=C1CSC(N=N2)=CC=C2C3=CC=CC=N3. InChi Code: InChI=1S/C17H15N3S/c1-13-6-2-3-7-14(13)12-21-17-10-9-16(19-20-17)15-8-4-5-11-18-15/h2-11H,12H2,1H3. InChi Key: DUUQLWDHNYFUPP-UHFFFAOYSA-N.