G6PDi-1

G6PDi-1 is a reversible and noncompetitive inhibitor of glucose-6-phosphate dehydrogenase (G6PDH, IC50 = 0.07 µM), the enzyme that converts G6P to 6-phosphogluconolactone using NADP+ as a cofactor in the first step of the pentose phosphate pathway. G6PDi-1 (10 and 50 µM) reduces NADPH levels in a variety of cultured cells, including red blood cells and T cells. It decreases colony formation of HCT116 cells when used at concentrations of 20 and 30 µM, an effect that can be rescued by the antioxidant N-acetyl cysteine. G6PDi-1 decreases the production of cytokines induced by phorbol 12-myristate 13-acetate (PMA, Cay-10008014) and ionomycin (Cay-10004974) in T cells and prevents PMA-induced oxidative burst in neutrophils.Formal Name: 4-((5-oxo-6,7,8,9-tetrahydro-5H-cyclohepta[d]pyrimidin-2-yl)amino)thiophene-2-carbonitrile. CAS Number: 2457232-14-1. Synonyms: Glucose-6-phosphate Dehydrogenase Inhibitor 1. Molecular Formula: C14H12N4OS. Formula Weight: 284.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 15 mg/ml, DMF:PBS (pH 7.2) (1:4): 0.20 mg/ml, DMSO: 10 mg/ml. lambdamax: 222, 246, 305 nm. SMILES: O=C(CCCC1)C2=C1N=C(NC3=CSC(C#N)=C3)N=C2. InChi Code: InChI=1S/C14H12N4OS/c15-6-10-5-9(8-20-10)17-14-16-7-11-12(18-14)3-1-2-4-13(11)19/h5,7-8H,1-4H2,(H,16,17,18). InChi Key: USWCHVCZOGGQQA-UHFFFAOYSA-N.