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G418 Sulfate is an aminoglycoside related to Gentamycin, used as a selective agent in bacterial genetics. It is toxic to yeast, bacteria, many plant and mammalian cells, helminths and protozoans. Appearance: White, crystalline powder, Solubility: Colorless, clear, complete, Potency: >620ug/mg, Suggested Working Concentrations: 100-800ug/ml Mammalian Selection: 400ug/ml, Maintenance: 200ug/ml, Absorbance (1mg/ml): 280nm: <0.015, 570nm: 0.10, Water (KF): <12%, Specific Rotation: +104° to +121ºC, pH: 5.0-6.0, Reconstitution: Reconstitute with water or ethanol to a desired stock concentration. Sterilize by filtration through a 0.2um filter, aliquot and freeze at -20°C. Storage and Stability: Powder: Store at 4ºC. Avoid moisture uptake, as this can decrease potency. Stable for 12 months after receipt., , Stock solution: Aliquot and store at -20°C. Stable for 3-6 months -at 20°C.
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