Fura-2 pentapotassium salt

Fura-2 pentapotassium salt
Item number Size Datasheet Manual SDS Delivery time Quantity Price
CDX-F0013-M001 1 mg - -

3 - 9 business days*

129.00€
 
Off-white solid to light yellow solid. Soluble in water or DMSO. Chemical. CAS: 113694-64-7.... more
Product information "Fura-2 pentapotassium salt"
Off-white solid to light yellow solid. Soluble in water or DMSO. Chemical. CAS: 113694-64-7. Formula: C29H22N3O14 . 5K. MW: 831.99. Fura-2 pentapotassium salt is a widely used UV-excitable fluorescent calcium indicator. It is a fluorophore chelating probe for Ca2+ used widely in biological systems to analyze intracellular calcium levels. It can be used to detect calcium in cells. A diagnostic shift in the excitation spectrum from 363nm to 335nm indicates FURA-2 chelation to Ca2+. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. Analysis of Ca2+ flux in and out of cells with Fura-2 introduces a powerful spectrographic handle to investigate and elucidate correlated biological events such as neuronal ion channel activation. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. It can also be used as a zinc indicator or measuring membrane potential. Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Fura-2 potassium salt is a cell-impermeable fluorescent probe for detection Ca2+ in a ratiometric mode. However, it may be loaded into cells via microinjection or scrape loading. Spectral data: lambdaEx 363nm, lambdaEm 512nm in water.
Keywords: Fura-2 . 5K
Supplier: Chemodex
Supplier-Nr: F0013

Properties

MW: 831,99 D
Formula: C29H22N3O14 . 5K
Purity: > 90% (HPLC)
Format: Solid

Database Information

CAS : 113694-64-7| Matching products

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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