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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
CDX-F0013-M001 | 1 mg | - | - |
3 - 9 business days* |
129.00€
|
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You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Off-white solid to light yellow solid. Soluble in water or DMSO. Chemical. CAS: 113694-64-7.... more
Product information "Fura-2 pentapotassium salt"
Off-white solid to light yellow solid. Soluble in water or DMSO. Chemical. CAS: 113694-64-7. Formula: C29H22N3O14 . 5K. MW: 831.99. Fura-2 pentapotassium salt is a widely used UV-excitable fluorescent calcium indicator. It is a fluorophore chelating probe for Ca2+ used widely in biological systems to analyze intracellular calcium levels. It can be used to detect calcium in cells. A diagnostic shift in the excitation spectrum from 363nm to 335nm indicates FURA-2 chelation to Ca2+. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. Analysis of Ca2+ flux in and out of cells with Fura-2 introduces a powerful spectrographic handle to investigate and elucidate correlated biological events such as neuronal ion channel activation. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. It can also be used as a zinc indicator or measuring membrane potential. Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Fura-2 potassium salt is a cell-impermeable fluorescent probe for detection Ca2+ in a ratiometric mode. However, it may be loaded into cells via microinjection or scrape loading. Spectral data: lambdaEx 363nm, lambdaEm 512nm in water.
Keywords: | Fura-2 . 5K |
Supplier: | Chemodex |
Supplier-Nr: | F0013 |
Properties
MW: | 831,99 D |
Formula: | C29H22N3O14 . 5K |
Purity: | > 90% (HPLC) |
Format: | Solid |
Database Information
CAS : | 113694-64-7| Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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