FSEN1

FSEN1 is an inhibitor of ferroptosis suppressor protein 1 (FSP1, IC50 = 313 nM). It is selective for FSP1 over NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1, IC50 = >100 µM). FSEN1 induces cell death in GPX4-/- H460 non-small cell lung cancer (NSCLC) cells (EC50 = 69.36 nM) but not control H460 cells expressing Cas9 (H460Cas9). It sensitizes A549, Huh7, HCC1143, U2OS, SU-DHL-5, and T98G cancer cells to ferroptosis induced by (1S,3R)-RSL3 (Cay-19288) when used at concentrations ranging from 10 to 1,000 nM. FSEN1 also sensitizes H460Cas9 cells, but not FSP1-/- H460 cells, to cell death induced by the ferroptosis inhibitors dihydroartemisinin (Cay-19846) or FINO2 (Cay-25096).Formal Name: 3-(4-bromophenyl)-6-[[4-(4-methoxyphenyl)-1-piperazinyl]methyl]-thiazolo[2,3-c]-1,2,4-triazole. CAS Number: 862808-01-3. Synonyms: Ferroptosis Sensitizer 1. Molecular Formula: C22H22BrN5OS. Formula Weight: 484.4. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 1 mg/ml, DMSO: Slightly soluble. lambdamax: 244, 289 nm. SMILES: COC(C=C1)=CC=C1N(CC2)CCN2CC3=CN4C(C5=CC=C(Br)C=C5)=NN=C4S3. InChi Code: InChI=1S/C22H22BrN5OS/c1-29-19-8-6-18(7-9-19)27-12-10-26(11-13-27)14-20-15-28-21(24-25-22(28)30-20)16-2-4-17(23)5-3-16/h2-9,15H,10-14H2,1H3. InChi Key: JGXIXBKKDUNARN-UHFFFAOYSA-N.