Fluorescein-5-thiosemicarbazide

Fluorescein-5-thiosemicarbazide (FTSC) is an amine-containing fluorescent probe with excitation/emission maxima of 495/517 nm, respectively. It can be reversibly coupled to aldehydes and ketones. FTSC has been used to fluorescently tag diverse molecules, including RNA, saccharides, sialylated glycoproteins, carbonylated proteins, and N-acetylneuraminic acid.Formal Name: N-(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-hydrazinecarbothioamide. CAS Number: 76863-28-0. Synonyms: FTSC. Molecular Formula: C21H15N3O5S. Formula Weight: 421.4. Purity: >95%. Emission: 517 nm. Excitation: 495 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 0.5 mg/ml, DMSO: 2 mg/ml, DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml. lambdamax: 226, 275 nm. SMILES: OC1=CC=C2C3(C4=CC=C(NC(NN)=S)C=C4C(O3)=O)C5=CC=C(O)C=C5OC2=C1. InChi Code: InChI=1S/C21H15N3O5S/c22-24-20(30)23-10-1-4-14-13(7-10)19(27)29-21(14)15-5-2-11(25)8-17(15)28-18-9-12(26)3-6-16(18)21/h1-9,25-26H,22H2,(H2,23,24,30). InChi Key: OIMMDIXLTJAVTF-UHFFFAOYSA-N.