Fluo-3 (potassium salt)

Fluo-3 (potassium salt) is a fluorescent calcium indicator commonly used in flow cytometry and cell-based experiments to detect changes in intracellular calcium levels. Its absorption maximum at 506 nm makes it compatible with excitation at 488 nm by argon-ion laser sources. Fluo-3 provides intense fluorescence upon binding calcium, detected at a maximum emission at 526 nm which can be monitored by FL1 (green, 525 nm band pass) sensors in flow cytometry.Formal Name: N-[2-[2-[2-[bis(carboxymethyl)amino]-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy]ethoxy]-4-methylphenyl]-N-(carboxymethyl)-glycine, pentapotassium salt. CAS Number: 853400-67-6. Molecular Formula: C36H25Cl2N2O13 . 5K. Formula Weight: 960.0. Purity: >90%. Emission: 526 nm. Excitation: 488 nm. Formulation: (Request formulation change), A solid. Solubility: Water: Soluble. SMILES: O=C1C(Cl)=CC(C(O2)=C1)=C(C3=CC=C(N(CC([O-])=O)CC([O-])=O)C(OCCOC4=C(N(CC([O-])=O)CC([O-])=O)C=CC(C)=C4)=C3)C5=C2C=C([O-])C(Cl)=C5.[K+].[K+].[K+].[K+].[K+]. InChi Code: InChI=1S/C36H30Cl2N2O13.5K/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36,,,,,/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,4. InChi Key: HESJPJDGZLKNAU-UHFFFAOYSA-I.