Fluo-3 AM

Fluo-3 is a fluorescent calcium indicator commonly used in flow cytometry (FC) and cell-based experiments to detect changes in intracellular calcium levels. Its absorption maximum at 506 nm makes it compatible with excitation at 488 nm by argon-ion laser sources. Fluo-3 provides intense fluorescence upon binding calcium, detected at a maximum emission at 526 nm which can be monitored by FL1 (green, 525 nm band pass) sensors in flow cytometry. Fluo-3 AM is an acetoxymethyl (AM) ester of fluo-3. This form, unlike fluo-3, is cell permeable and readily taken up by living cells, whereupon the AM moiety is enzymatically removed to produce fluo-3.Formal Name: N-[4-[6-[(acetyloxy)methoxy]-2,7-dichloro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine, (acetyloxy)methyl ester. CAS Number: 121714-22-5. Synonyms: Fluo-3 Acetoxymethyl ester. Molecular Formula: C51H50Cl2N2O23. Formula Weight: 1129.9. Purity: >90%. Emission: 526 nm. Excitation: 488 nm. Formulation: (Request formulation change), A solution in acetonitrile. Solubility: Acetonitrile: 2 mg/ml, DMSO: 0.9 mg/ml. lambdamax: 214, 369, 465 nm. SMILES: ClC1=C(OCOC(C)=O)C=C(O2)C(C(C3=CC(OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(C)=C4)=C(C=C3)N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(C2=CC5=O)C=C5Cl)=C1. InChi Code: InChI=1S/C51H50Cl2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73. InChi Key: PTPUOMXKXCCSEN-UHFFFAOYSA-N.