Everolimus

Everolimus is an immunosuppressant analog of rapamycin (sirolimus) and inhibits growth factor-mediated proliferation of hematopoietic and nonhematopoietic cells. Everolimus shows a potent inhibitory effect on posttransplant lymphoproliferative disorder (PTLD)-derived cells in vitro and in vivo, in a dose range that can prevent allograft rejection, and may demonstrate activity in both the prevention and treatment of PTLDs in transplant patients. Cis-Isomer (HPLC): <15%, Melting Point: 90-115°C, Solubility: Soluble in DMSO at 100mg/ml, soluble in ethanol at 100mg/ml, very poorly soluble in water, maximum solubility in plain water is estimated to be about 1-10uM, buffers, serum, or other additives may increase or decrease the aqueous solubility. Elemental Analysis: Calculated:, C - 65.51%, H - 8.76%, N - 1.44%, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with DMSO or EtOH. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.