DUB-IN-1

DUB-IN-1 is an inhibitor of ubiquitin-specific protease 8 (USP8, IC50 = 0.85 µM). It is selective for USP8 over USP7 (IC50 = >100 µM). It inhibits the proliferation of LN-229, U87MG, and T98G glioblastoma cells when used at concentrations ranging from 200 to 800 nM. DUB-IN-1 (500 and 800 nM) reduces the migration and stemness of LN-229 and T98G cells and sensitizes both to ionizing radiation. It also reduces the viability of a variety of esophageal squamous cell carcinoma (ESCC) cells (IC50s = 1.58-2.14 µM) and induces apoptosis, autophagy, and cell cycle arrest at the G2/M phase in KYSE-450 and KYSE-30 ESCC cells.Formal Name: 9-[(phenylmethoxy) imino]-9H-indeno[1,2-b] pyrazine-2,3-dicarbonitrile. CAS Number: 924296-18-4. Synonyms: Deubiquitinase-IN-1, Deubiquitinase Inhibitor 1, DUB Inhibitor 1, DUBs-IN-1, DUBs Inhibitor 1. Molecular Formula: C20H11N5O. Formula Weight: 337.3. Purity: >95%. Formulation: (Request formulation change), A solid. Solubility: DMSO: Soluble: >10 mg/ml. SMILES: N#CC(N=C12)=C(C#N)N=C2C3=C(C=CC=C3)C1=NOCC4=CC=CC=C4. InChi Code: InChI=1S/C20H11N5O/c21-10-16-17(11-22)24-20-18(23-16)14-8-4-5-9-15(14)19(20)25-26-12-13-6-2-1-3-7-13/h1-9H,12H2. InChi Key: GKOWDIBLCDZJHF-UHFFFAOYSA-N.