DTNB

DTNB
Item number Size Datasheet Manual SDS Delivery time Quantity Price
CDX-D0156-G001 1 g - -

3 - 9 business days*

37.00€
CDX-D0156-G005 5 g - -

3 - 9 business days*

85.00€
CDX-D0156-G025 25 g - -

3 - 9 business days*

191.00€
 
Yellow powder. Soluble in ethanol, dioxane or phosphate buffer. A sensitive reagent for measuring... more
Product information "DTNB"
Yellow powder. Soluble in ethanol, dioxane or phosphate buffer. A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. Used to characterize reactive thiol groups and photometric determination of thiols and for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood. It can also measure the number of thiol groups on proteins. Through reaction with aliphatic thiol groups a mixed disulfide of protein thiol and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group is being formed. DTNB has little absorbance. Reaction with -SH groups on proteins (from any solvent accessible Cys) under mild alkaline conditions (pH 7-8) produces the 2-nitro-5-thiobenzoate anion, which gives an intense yellow color with an absorption maximum at 409.5nm (Extinction coefficient: 14150 M-1*cm-1). Sensitive to various buffer ions, therefore, the extinction coefficient used to calculate the number of sulfhydryl groups must be matched to the reaction conditions. In case the thiol groups are in disulfide bonds, they must be reduced under anaerobic conditions prior to reaction with DTNB.
Keywords: Ellman's Reagent, 5,5'-Dithiobis(2-nitrobenzoic acid)
Supplier: Chemodex
Supplier-Nr: D0156

Properties

Application: Free sulfhydryl content determination
MW: 396,35 D
Formula: C14H8N2O8S2
Purity: >98%
Format: Solid

Database Information

CAS : 69-78-3| Matching products

Handling & Safety

Storage: -20°C
Shipping: +20°C (International: +20°C)
GHS Hazard Pictograms:
H Phrases: H315, H319, H335
P Phrases: P261, P305+P351+P338
Caution
Our products are for laboratory research use only: Not for administration to humans!
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