DPTIP (hydrochloride)

DPTIP is an inhibitor of neutral sphingomyelinase 2 (nSMase2, IC50 = 30 nM). It is selective for nSMase2 over alkaline phosphatase (ALP) and acid sphingomyelinase (IC50s = >100 µM for both). DPTIP reduces viral yield in Vero and HeLa cells infected with West Nile virus (EC50s = 0.26 and 2.81 µM, respectively) or Zika virus (EC50s = 1.56 and 1.84 µM, respectively). It inhibits the secretion of extracellular vesicles from primary mouse astrocytes activated by FBS withdrawal in a concentration-dependent manner and prevents serum deprivation-induced astrocyte activation in primary rat astrocytes when used at a concentration of 10 µM. DPTIP (10 mg/kg) reduces IL-1beta-induced extracellular vesicle release from astrocytes and decreases neutrophil infiltration to the brain in a model of inflammation-induced brain injury using GFAP-GFP mice. It reduces hepatic levels of chemokine (C-C motif) ligand 2 (Ccl2), Tnf-alpha, Il-6, and Il-1beta in the same model.Formal Name: 2,6-dimethoxy-4-[4-phenyl-5-(2-thienyl)-1H-imidazol-2-yl]-phenol, monohydrochloride. CAS Number: 2361799-64-4. Molecular Formula: C21H18N2O3S . HCl. Formula Weight: 414.9. Purity: >95%. Formulation: (Request formulation change), A solid. Solubility: Acetonitrile: Slightly Soluble: 0.1-1 mg/ml, DMSO: Sparingly Soluble: 1-10 mg/mL. SMILES: OC1=C(OC)C=C(C2=NC(C3=CC=CC=C3)=C(C4=CC=CS4)N2)C=C1OC.Cl. InChi Code: InChI=1S/C21H18N2O3S.ClH/c1-25-15-11-14(12-16(26-2)20(15)24)21-22-18(13-7-4-3-5-8-13)19(23-21)17-9-6-10-27-17,/h3-12,24H,1-2H3,(H,22,23),1H. InChi Key: JXCSNAONSJHTTR-UHFFFAOYSA-N.