DiBAC4(3)

DiBAC4(3) is a negatively charged fluorescent membrane potential indicator. Upon membrane depolarization, DiBAC4(3) enters the cytosol, binds to lipid membranes and intracellular proteins, and displays excitation/emission maxima of 490/516 nm, respectively. It has been used to measure the membrane potential of live or fixed mammalian and bacterial cells by flow cytometry or fluorescence microscopy.Formal Name: 1,3-dibutyl-5-[3-(1,3-dibutylhexahydro-2,4,6-trioxo-5-pyrimidinyl)-2-propen-1-ylidene]-2,4,6(1H,3H,5H)-pyrimidinetrione. CAS Number: 70363-83-6. Synonyms: Bis(1,3-Dibutylbarbituric Acid) Trimethine Oxonol. Molecular Formula: C27H40N4O6. Formula Weight: 516.6. Purity: >95%. Emission: 516 nm. Excitation: 490 nm. Formulation: (Request formulation change), A solid. Solubility: DMF: 10 mg/ml, DMSO: 15 mg/ml, DMSO:PBS (pH 7.2) (1:2): 0.33 mg/ml, Ethanol: 5 mg/ml. lambdamax: 495 nm. SMILES: O=C1/C(C(N(CCCC)C(N1CCCC)=O)=O)=C/C=CC2C(N(CCCC)C(N(CCCC)C2=O)=O)=O. InChi Code: InChI=1S/C27H40N4O6/c1-5-9-16-28-22(32)20(23(33)29(26(28)36)17-10-6-2)14-13-15-21-24(34)30(18-11-7-3)27(37)31(25(21)35)19-12-8-4/h13-15,20H,5-12,16-19H2,1-4H3. InChi Key: ATJCYSYHWGQAET-UHFFFAOYSA-N.