CRANAD 28

CRANAD 28 is a curcumin derivative and non-conjugated fluorescent affinity probe for the detection of amyloid-beta (Abeta) in vitro and in vivo that has excitation/emission spectra of 498/578 nm, respectively. It binds to various forms of Abeta, including Abeta40 monomers and aggregates, as well as Abeta42 monomers, dimers, and oligomers (Kds = 68.8, 52.4, 159.7, 162.9, and 85.7 nM, respectively). When bound to Abeta, the fluorescence intensity decreases, in contrast to similar curcumin-based fluorescent probes. CRANAD 28 labels amyloid plaques and cerebral amyloid angiopathy both in APP/PS1 mouse brain sections and in live mice following i.v. administration. It also inhibits copper-induced and naturally occurring Abeta crosslinking.CAS Number: 1623747-97-6. Molecular Formula: C27H23BF2N4O2. Formula Weight: 484.3. Purity: >98%. Emission: 578 nm. Excitation: 498 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 2 mg/ml, DMSO: 2 mg/ml, DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml. lambdamax: 245, 479 nm. SMILES: [F-][B+3]1([F-])[O]=C([CH-]C(/C=C/C2=C(C)N(C3=CC=CC=C3)N=C2)=[O]1)/C=C/C4=C(C)N(C5=CC=CC=C5)N=C4. InChi Code: InChI=1S/C27H23BF2N4O2/c1-20-22(18-31-33(20)24-9-5-3-6-10-24)13-15-26-17-27(36-28(29,30)35-26)16-14-23-19-32-34(21(23)2)25-11-7-4-8-12-25/h3-19H,1-2H3/b15-13+,16-14+. InChi Key: DDFFGVTUEQJYQK-WXUKJITCSA-N.