C29

C29 is an inhibitor of toll-like receptor 2 (TLR2) signaling. It inhibits synthetic and bacterial TLR2 agonist-induced TLR2/1 and TLR2/6 signaling in HEK-293T cells expressing human TLR2 (HEK-TLR2) and THP-1 cells when used at a concentration of 50 µM. C29 also inhibits TLR2/1 signaling in primary murine macrophages without inducing cytotoxicity. It decreases the expression of IL-8 induced by the synthetic bacterial lipopeptide TLR2 agonist Pam3CSK4 (Cay-24126) in HEK-TLR2 cells. C29 (150 µM) inhibits the Pam3CSK4-induced interaction between TLR2 and the adapter protein MyD88, blocks MAPK activation, and decreases NF-kappaB activation and IkappaBalpha degradation in THP-1 cells.Formal Name: 3-[[(2-hydroxy-3-methoxyphenyl)methylene]amino]-2-methyl-benzoic acid. CAS Number: 363600-92-4. Synonyms: TLR2-IN-C29. Molecular Formula: C16H15NO4. Formula Weight: 285.3. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 10 mg/ml, DMSO: 11 mg/ml, Ethanol: 0.5 mg/ml, PBS (pH 7.2): 0.3 mg/ml. lambdamax: 226, 277, 320 nm. SMILES: O=C(O)C1=CC=CC(/N=C/C2=C(O)C(OC)=CC=C2)=C1C. InChi Code: InChI=1S/C16H15NO4/c1-10-12(16(19)20)6-4-7-13(10)17-9-11-5-3-8-14(21-2)15(11)18/h3-9,18H,1-2H3,(H,19,20)/b17-9+. InChi Key: WTGMGRFVBFDHGQ-RQZCQDPDSA-N.