Boc-Lys(Ac)-AMC

Boc-Lys(Ac)-AMC is a fluorescent substrate for histone deacetylases (HDACs). Following deacetylation by an HDAC, Boc-Lys-AMC is cleaved by trypsin and 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify HDAC activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.Formal Name: N-[(1S)-5-(acetylamino)-1-[[(4-methyl-2-oxo-2H-1-benzopyran-7-yl)amino]carbonyl]pentyl]-carbamic acid, 1,1-dimethylethyl ester. CAS Number: 233691-67-3. Molecular Formula: C23H31N3O6. Formula Weight: 445.5. Purity: >98%. Emission: 440-460 nm. Excitation: 340-360 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 25 mg/ml, Ethanol: 3 mg/ml. lambdamax: 211, 229, 328 nm. SMILES: O=C1C=C(C)C2=CC=C(NC([C@@H](NC(OC(C)(C)C)=O)CCCCNC(C)=O)=O)C=C2O1. InChi Code: InChI=1S/C23H31N3O6/c1-14-12-20(28)31-19-13-16(9-10-17(14)19)25-21(29)18(8-6-7-11-24-15(2)27)26-22(30)32-23(3,4)5/h9-10,12-13,18H,6-8,11H2,1-5H3,(H,24,27)(H,25,29)(H,26,30)/t18-/m0/s1. InChi Key: SUTRDULVNIPNLW-SFHVURJKSA-N.