Bobcat 339

Bobcat 339 is a cytosine derivative that was originally found to be an inhibitor of ten-eleven translocation methylcytosine dioxygenase 1 (TET1) and TET2 (IC50s = 33 and 73 µM, respectively, in cell-free assays). However, it was found that the TET inhibitory activity was due to residual copper in the Bobcat 339 preparation used for the experiments. Copper-containing Bobcat 339 (10 µM) also reduces DNA 5-hydroxymethylcytosine (5-hmC) levels in HT22 hippocampal neurons, but copper-free preparations of Bobcat 339 (50 µM) do not reduce DNA 5-hmC levels in Hep3B cells.Formal Name: 4-amino-1-[1,1'-biphenyl]-3-yl-5-chloro-2(1H)-pyrimidinone. CAS Number: 2280037-51-4. Synonyms: BC339. Molecular Formula: C16H12ClN3O. Formula Weight: 297.7. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 1 mg/ml, DMSO: 1 mg/ml, DMSO:PBS (pH 7.2) (1:8): 0.11 mg/ml. lambdamax: 250 nm. SMILES: O=C1N=C(C(Cl)=CN1C2=CC=CC(C3=CC=CC=C3)=C2)N. InChi Code: InChI=1S/C16H12ClN3O/c17-14-10-20(16(21)19-15(14)18)13-8-4-7-12(9-13)11-5-2-1-3-6-11/h1-10H,(H2,18,19,21). InChi Key: QMGYGOOYCNTEQO-UHFFFAOYSA-N.