bis-ANS (potassium salt)

bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation. Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings. bis-ANS has an excitation maxima of 390 nm. It has an emission maximum of 523 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein, for example, when bound to intestinal fatty acid binding protein (FAPB2) it has emission maxima of 484-496 nm. bis-ANS has been used to label mechanically damaged neurons in acute brain slices. It also potently inhibits microtubule assembly.Formal Name: 4,4'-bis(phenylamino)-[1,1'-binaphthalene]-5,5'-disulfonic acid, dipotassium salt. CAS Number: 912-60-7. Synonyms: 4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic Acid. Molecular Formula: C32H22N2O6S2 . 2K. Formula Weight: 672.9. Purity: >95%. Emission: 523 (free), 496 (bound) nm. Excitation: 390 (free), 484 (bound) nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, Ethanol: Slightly soluble, PBS (pH 7.2): 5 mg/ml. lambdamax: 222, 275, 395 nm. SMILES: [O-]S(C1=CC=CC2=C1C(NC3=CC=CC=C3)=CC=C2C4=C(C=CC=C5S([O-])(=O)=O)C5=C(NC6=CC=CC=C6)C=C4)(=O)=O.[K+].[K+]. InChi Code: InChI=1S/C32H24N2O6S2.2K/c35-41(36,37)29-15-7-13-25-23(17-19-27(31(25)29)33-21-9-3-1-4-10-21)24-18-20-28(34-22-11-5-2-6-12-22)32-26(24)14-8-16-30(32)42(38,39)40,,/h1-20,33-34H,(H,35,36,37)(H,38,39,40),,/q,2*+1/p-2. InChi Key: VWLWTJHKQHRTNC-UHFFFAOYSA-L.