BCIP (p-toluidine salt)

BCIP is a colorimetric substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry (IHC) or Western blot (WB).Formal Name: 5-bromo-4-chloro-1H-indol-3-ol 3-(dihydrogen phosphate) compd. with 4-methylbenzenamine. CAS Number: 6578-06-9. Synonyms: 5-Bromo-4-chloro-3-indoyl phosphate. Molecular Formula: C8H6BrClNO4P . C7H9N. Formula Weight: 433.6. Purity: >95%. Formulation: (Request formulation change), A solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, DMSO:PBS (pH 7.2) (1:4): 0.2 mg/ml. lambdamax: 232 nm. SMILES: O=P(O)(OC1=CNC2=C1C(Cl)=C(Br)C=C2)O.NC3=CC=C(C)C=C3. InChi Code: InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14,1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14),2-5H,8H2,1H3. InChi Key: QEIFSLUFHRCVQL-UHFFFAOYSA-N.