ADHP

ADHP is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2O2. This colorless, non-fluorescent reagent reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl sample.Formal Name: 1-(3,7-dihydroxy-10H-phenoxazin-10-yl)-ethanone. CAS Number: 119171-73-2. Synonyms: A 6550, 10-Acetyl-3,7-dihydroxyphenoxazine, Amplex Red(R). Molecular Formula: C14H11NO4. Formula Weight: 257.2. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 25 mg/ml, DMSO: 25 mg/ml, DMSO:PBS (pH 7.2)1:5: 0.15 mg/ml, Ethanol: 1.5 mg/ml. lambdamax: 250, 285 nm. SMILES: Oc1ccc2c(c1)Oc1cc(O)ccc1N2C(=O)C. InChi Code: InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3. InChi Key: PKYCWFICOKSIHZ-UHFFFAOYSA-N.