Ac-IEPD-AMC (trifluoroacetate salt)

Ac-IEPD-AMC is a fluorogenic substrate for granzyme B. Upon enzymatic cleavage by granzyme B, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify granzyme B activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.Formal Name: N-acetyl-L-isoleucyl-L-alpha-glutamyl-L-prolyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-alpha-asparagine, trifluoroacetate salt. Synonyms: Acetyl-Ile-Glu-Pro-Asp-7-amino-4-Methylcoumarin, Ac-Ile-Glu-Pro-Asp-AMC. Molecular Formula: C32H41N5O11. XCF3COOH. Formula Weight: 671.7. Purity: >95%. Emission: 440-460 nm. Excitation: 340-360 nm. Formulation: (Request formulation change), A solid. Solubility: DMSO: 1 mg/ml. SMILES: O=C(C)N[C@H](C(N[C@H](C(N1CCC[C@H]1C(N[C@@H](CC(O)=O)C(NC2=CC=C(C(C)=CC(O3)=O)C3=C2)=O)=O)=O)CCC(O)=O)=O)[C@@H](C)CC.FC(F)(C(O)=O)F. InChi Code: InChI=1S/C32H41N5O11.C2HF3O2/c1-5-16(2)28(33-18(4)38)31(46)35-21(10-11-25(39)40)32(47)37-12-6-7-23(37)30(45)36-22(15-26(41)42)29(44)34-19-8-9-20-17(3)13-27(43)48-24(20)14-19,3-2(4,5)1(6)7/h8-9,13-14,16,21-23,28H,5-7,10-12,15H2,1-4H3,(H,33,38)(H,34,44)(H,35,46)(H,36,45)(H,39,40)(H,41,42),(H,6,7)/t16-,21-,22-,23-,28-,/m0./s1. InChi Key: DPJJHJBBZSQESC-WQMVBLBISA-N.