ABTS (ammonium salt)

ABTS is a radical cation and a substrate of peroxidases, including horseradish peroxidase (HRP). It has commonly been used to assess antioxidant capacity in the Trolox equivalent antioxidant capacity (TEAC) assay. It has a blue color in the presence of sodium persulfate or metmyoglobin but decolorizes upon incubation with antioxidants, and the antioxidant capacity can be determined spectrophotometrically. ABTS has also been used as an enzyme substrate in ELISAs. Upon incubation with a peroxidase, it produces a soluble green product that can be quantified by colorimetric detection at 405 nm.Formal Name: 2,2'-(1,2-hydrazinediylidene)bis[3-ethyl-2,3-dihydro-6-benzothiazolesulfonic acid, diammonium salt. CAS Number: 30931-67-0. Molecular Formula: C18H16N4O6S4 . 2NH4. Formula Weight: 548.7. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 1 mg/ml, DMSO: 10 mg/ml, PBS (pH 7.2): 3 mg/ml. lambdamax: 224, 346 nm. SMILES: [O-]S(C1=CC=C(N(CC)/C(S2)=N/N=C3SC(C=C(S([O-])(=O)=O)C=C4)=C4N\3CC)C2=C1)(=O)=O.[NH4+].[NH4+]. InChi Code: InChI=1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18,,/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28),2*1H3/b19-17-,20-18-,,. InChi Key: OHDRQQURAXLVGJ-AXMZSLBLSA-N.