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Product information "5-Carboxytetramethylrhodamine succinimidyl ester"
5-Carboxytetramethylrhodamine succinimidyl ester (5-TAMRA-SE) is an amine-reactive fluorescent probe. It has been used in double site-directed peptide modification to label proteinase substrates for use in FRET assays. It quenches lucifer yellow CH (Cay-25573) fluorescence by greater than 90% in uncleaved proteinase substrates, which allows for the detection of enzyme-cleaved substrates by an increase in fluorescence intensity. It has also been used in the synthesis of fluorescent derivatives of a variety of compounds, including the antibiotic ampicillin (Cay-14417), nucleotide diphosphate uridine-5'-diphosphate (UDP, Cay-18137), and the progesterone receptor antagonist RU486 (mifepristone, Cay-10006317). 5-TAMRA-SE displays excitation maxima ranging from 540 to 560 nm and an emission maximum of 580 nm.Formal Name: 9-[2-carboxy-4-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-3,6-bis(dimethylamino)-xanthylium, inner salt. CAS Number: 150810-68-7. Synonyms: 5-TAMRA-SE. Molecular Formula: C29H25N3O7. Formula Weight: 527.5. Purity: >90%. Emission: 580 nm. Excitation: 540-560 nm. Formulation: (Request formulation change), A solid. Solubility: DMF: 2 mg/ml, DMSO: 1.4 mg/ml, Ethanol: 0.2 mg/ml, PBS (pH 7.2): 0.25 mg/ml. lambdamax: 357, 545 nm. SMILES: [O-]C(C(C=C(C(ON1C(CCC1=O)=O)=O)C=C2)=C2C3=C4C=CC(N(C)C)=CC4=[O+]C5=C3C=CC(N(C)C)=C5)=O. InChi Code: InChI=1S/C29H25N3O7/c1-30(2)17-6-9-20-23(14-17)38-24-15-18(31(3)4)7-10-21(24)27(20)19-8-5-16(13-22(19)28(35)36)29(37)39-32-25(33)11-12-26(32)34/h5-10,13-15H,11-12H2,1-4H3. InChi Key: VWFRSNKRTNUMET-UHFFFAOYSA-N.
Keywords:
5-TAMRA-SE, 9-[2-carboxy-4-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-3,6-bis(dimethylamino)-xanthylium, inner salt
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