5(6)-Carboxyfluorescein diacetate

5(6)-Carboxyfluorescein diacetate (CFDA) is a fluorescent probe used to stain cells with intact membranes for analysis in applications such as flow cytometry or fluorescence microscopy. It has relatively slow cellular efflux with a half-life of ~94 minutes and displays fluorescence with excitation/emission maxima of 494/521 nm.Formal Name: 3',6'-bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid. CAS Number: 124387-19-5. Synonyms: CFDA. Molecular Formula: C25H16O9. Formula Weight: 460.4. Purity: >98% (mixture of isomers). Emission: 521 nm. Excitation: 494 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 50 mg/mL, DMF:PBS (pH 7.2) (1:4): 0.2 mg/mL, DMSO: 30 mg/mL. lambdamax: 220, 291 nm. SMILES: O=C(O1)C2=C(C=CC(C(O)=O)=C2)C31C4=CC=C(OC(C)=O)C=C4OC5=C3C=CC(OC(C)=O)=C5.O=C(O6)C7=C(C=C(C(O)=O)C=C7)C86C9=CC=C(OC(C)=O)C=C9OC%10=C8C=CC(OC(C)=O)=C%10. InChi Code: InChI=1S/2C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25,1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h2*3-11H,1-2H3. InChi Key: ZCFZHZUTXKEHLO-UHFFFAOYSA-N.