5(6)-Carboxy-2',7'-dichlorofluorescein diacetate

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe. Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2',7',-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation/emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production.Formal Name: 3',6'-bis(acetyloxy)-2',7'-dichloro-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid. CAS Number: 127770-45-0. Synonyms: CDFDA. Molecular Formula: C25H14Cl2O9. Formula Weight: 529.3. Purity: >85% (mixture of isomers). Emission: 530 nm. Excitation: 504 nm. Formulation: (Request formulation change), A solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml. SMILES: O=C(O1)C2=C(C=CC(C(O)=O)=C2)C31C4=CC(Cl)=C(OC(C)=O)C=C4OC5=C3C=C(Cl)C(OC(C)=O)=C5.O=C(O6)C7=C(C=C(C(O)=O)C=C7)C86C9=CC(Cl)=C(OC(C)=O)C=C9OC%10=C8C=C(Cl)C(OC(C)=O)=C%10. InChi Code: InChI=1S/2C25H14Cl2O9/c1-10(28)33-21-8-19-15(6-17(21)26)25(14-4-3-12(23(30)31)5-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19,1-10(28)33-21-8-19-15(6-17(21)26)25(14-5-12(23(30)31)3-4-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19/h2*3-9H,1-2H3,(H,30,31). InChi Key: LZMPJBOMBCYYMP-UHFFFAOYSA-N.