4-Methylumbelliferyl-alpha-L-Iduronide 2-sulfate (sodium salt)

4-Methylumbelliferyl-alpha-L-iduronide 2-sulfate (4-MU-alpha-IdoA 2-sulfate) is a fluorogenic substrate of alpha-L-iduronidase that has been used in an assay to detect Hurler syndrome. It is also used as a substrate for iduronate-2-sulfatase in tests for Hunter disease. For these latter tests, the initial enzymatic product, 4-MU-alpha-IdoA (Cay-19543) can be measured by mass spectrometry, or it can be hydrolyzed with alpha-L-iduronidase to liberate the fluorophore 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.Formal Name: (2R,3S,4S,5R,6S)-3,4-dihydroxy-6-((4-methyl-2-oxo-2H-chromen-7-yl)oxy)-5-(sulfonatooxy)tetrahydro-2H-pyran-2-carboxylate, disodium salt. Synonyms: 4-Methylumbelliferyl-alpha-L-Idopyranosiduronic Acid 2-sulfate, 4-MU-alpha-IdoA 2-sulfate. Molecular Formula: C16H14O12S . 2Na. Formula Weight: 476.3. Purity: >98% (mixture of isomers). Emission: 445-454 nm. Excitation: 330 (pH 4.6), 370 (pH 7.4), 385 (pH 10.4). Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, PBS (pH 7.2): 10 mg/ml. lambdamax: 319 nm. SMILES: O=C1C=C(C)C2=C(C=C(O[C@H]3[C@H](OS([O-])(=O)=O)[C@@H](O)[C@H](O)[C@H](C([O-])=O)O3)C=C2)O1.[Na+].[Na+]. InChi Code: InChI=1S/C16H16O12S.2Na/c1-6-4-10(17)26-9-5-7(2-3-8(6)9)25-16-14(28-29(22,23)24)12(19)11(18)13(27-16)15(20)21,,/h2-5,11-14,16,18-19H,1H3,(H,20,21)(H,22,23,24),,/q,2*+1/p-2/t11-,12-,13+,14+,16+,,/m0../s1. InChi Key: BGWMBPGUPUSUIU-CEWNINSASA-L.