4-Methylumbelliferyl-2-acetamido-2-deoxy-beta-D-Glucopyranoside

4-Methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside is a fluorogenic substrate for beta-hexosaminidases. Upon enzymatic cleavage by beta-hexosaminidases, 4-methylumbelliferone (4-?U) is released and its fluorescence can be used to quantify beta-hexosaminidase activity. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high pH (7.12-10.3), respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. 4-Methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside has been used to quantify beta-hexosaminidase activity in serum or leukocytes from patients with GM2 gangliosidoses such as Tay-Sachs disease, which is characterized by defects in the alpha subunit of beta-hexosaminidase.Formal Name: 7-[[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]oxy]-4-methyl-2H-1-benzopyran-2-one. CAS Number: 37067-30-4. Synonyms: 4-Methylumbelliferyl-N-acetyl-beta-Glucosaminide, 4-?U-2-acetamido-2-deoxy-beta-D-Glucopyranoside. Molecular Formula: C18H21NO8. Formula Weight: 379.4. Purity: >98%. Emission: 455 and 445 nm at low and high pH, respectively. Excitation: 320 and 360 nm at low and high pH, respectively. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 5 mg/ml, DMSO: 30 mg/ml. SMILES: O=C(C)N[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](CO)[C@@H](O)[C@@H]1O. InChi Code: InChI=1S/C18H21NO8/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21)/t13-,15-,16-,17-,18-/m1/s1. InChi Key: QCTHLCFVVACBSA-JVNHZCFISA-N.