15,16-Dihydrotanshinone I

15,16-Dihydrotanshinone I (DHTS) is a diterpene tanshinone that has been found in the roots of S. miltiorrhiza with diverse biological activities. DHTS inhibits RHL-2H3 mast cell degranulation with an IC50 value of 14.3 µM and reduces the tyrosine phosphorylation of phospholipase Cgamma2 (PLCgamma2) and ERK. It prevents HuR binding to RNA in a cell-free assay (Ki = 3.74 nM) and inhibits the production of TNF mRNA and protein in MCF-7 cells. DHTS reduces the viability of MCF-7, MDA-MB-231, and SK-BR-3 breast cancer cells with IC50 values of 0.84, 0.92, and 1.2 µM, respectively. It also reduces collagen-induced aggregation of washed rabbit platelets (IC50 = 8.7 µM). In vivo, DHTS (25 mg/kg per day) reduces tumor growth in an HL-60 leukemia mouse xenograft model by 68% relative to control without decreasing body weight. In mice with scopolamine-induced learning and memory impairment, DHTS (2-4 mg/kg, p.o.) increases latency to step-through in a passive avoidance test by approximately 3- to 4-fold, and it inhibits acetylcholinesterase in brain homogenate (IC50 = 25 µM).Formal Name: (1R)-1,2-dihydro-1,6-dimethyl-phenanthro[1,2-b]furan-10,11-dione. CAS Number: 87205-99-0. Synonyms: DHTS, Dihydrotanshinone I. Molecular Formula: C18H14O3. Formula Weight: 278.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 0.2 mg/ml, DMSO: 0.2 mg/ml. lambdamax: 215, 241, 290 nm. SMILES: O=C(C1=C2C(C(C)=CC=C2)=CC=C1C3=C4[C@@H](C)CO3)C4=O. InChi Code: InChI=1S/C18H14O3/c1-9-4-3-5-12-11(9)6-7-13-15(12)17(20)16(19)14-10(2)8-21-18(13)14/h3-7,10H,8H2,1-2H3/t10-/m0/s1. InChi Key: HARGZZNYNSYSGJ-JTQLQIEISA-N. Origin: Plant/Salvia miltiorrhiza.