1-NA-PP1

1-NA-PP1 is a reversible, cell-permeable inhibitor of Src-family tyrosine kinases that have been mutated, by a single base substitution, to become 'analog sensitive' (as), as compared to the wild-type kinase. 1-NA-PP1 was first developed to optimally inhibit v-Src-as1, with an I338G substitution, preferentially over v-Src (IC50 = 1.5 nM versus 1.0 µM, respectively). The homologous mutation in other kinases generated similar analog sensitivity (e.g., IC50 = 1.5 nM for c-Fyn-as1 versus 0.6 µM for c-Fyn, 7.0 nM for c-Abl-as2 versus 0.6 µM for c-Abl, 15 nM for Cdk2-as1 versus 18 µM for Cdk2). This approach has been used to elucidate functions of several kinases in both mammalian and yeast systems.Formal Name: 1-(1,1-dimethylethyl)-3-(1-naphthalenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine. CAS Number: 221243-82-9. Synonyms: 1-Naphthyl-PP1, PP1 Analog. Molecular Formula: C19H19N5. Formula Weight: 317.4. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMF:PBS (pH 7.2) (1:5): 0.15 mg/ml, DMSO: 1 mg/ml, Ethanol: 2 mg/ml. lambdamax: 214, 286 nm. SMILES: NC1=C(C(C2=C(C=CC=C3)C3=CC=C2)=NN4C(C)(C)C)C4=NC=N1. InChi Code: InChI=1S/C19H19N5/c1-19(2,3)24-18-15(17(20)21-11-22-18)16(23-24)14-10-6-8-12-7-4-5-9-13(12)14/h4-11H,1-3H3,(H2,20,21,22). InChi Key: XSHQBIXMLULFEV-UHFFFAOYSA-N.