Rat SRD5a1 (Steroid 5 Alpha Reductase 1) ELISA Kit

Rat SRD5a1 (Steroid 5 Alpha Reductase 1) ELISA Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ELK-ELK7934.48 48 wells - -

10 - 14 business days*

365.00€
ELK-ELK7934.96 96 wells - -

10 - 14 business days*

522.00€
 
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate... more
Product information "Rat SRD5a1 (Steroid 5 Alpha Reductase 1) ELISA Kit"
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SRD5a1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SRD5a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SRD5a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SRD5a1 in the samples is then determined by comparing the OD of the samples to the standard curve.. Detection Range: 0.16-10 ng/mL. Sensitivity: 0.058 ng/mL. Assay Type: Sandwich. Standard: 10 ng/mL. Sample Type: Tissue homogenates, cell lysates and other biological fluids. Assay duration: 3.5h
Keywords: S5AR 1, SR type 1, EC=1.3.1.22, Steroid 5-alpha-reductase 1, 3-oxo-5-alpha-steroid 4-dehydrogenase 1
Supplier: ELK Biotechnology
Supplier-Nr: ELK7934

Properties

Application: ELISA
Species reactivity: rat
Format: Solid Phase

Handling & Safety

Storage: vT
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow. more
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "Rat SRD5a1 (Steroid 5 Alpha Reductase 1) ELISA Kit"
Write a review
or to review a product.
Viewed