Rat IL10 (Interleukin 10) ELISA Kit

Rat IL10 (Interleukin 10) ELISA Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ELK-ELK1144.48 48 wells - -

10 - 14 business days*

303.00€
ELK-ELK1144.96 96 wells - -

10 - 14 business days*

429.00€
 
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate... more
Product information "Rat IL10 (Interleukin 10) ELISA Kit"
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL10 in the samples is then determined by comparing the OD of the samples to the standard curve.. Detection Range: 7.82-500 pg/mL. Sensitivity: 3.1 pg/mL. Assay Type: Sandwich. Standard: 500 pg/mL. Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay duration: 3.5h
Keywords: CSIF, Il10, Il-10, IL-10, Interleukin-10, Cytokine synthesis inhibitory factor
Supplier: ELK Biotechnology
Supplier-Nr: ELK1144

Properties

Application: ELISA
Species reactivity: rat
Format: Solid Phase

Handling & Safety

Storage: vT
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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