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Rat GDF15 (Growth Differentiation Factor 15) ELISA Kit
Product information "Rat GDF15 (Growth Differentiation Factor 15) ELISA Kit"
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GDF15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDF15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GDF15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GDF15 in the samples is then determined by comparing the OD of the samples to the standard curve.. Detection Range: 15.63-1000 pg/mL. Sensitivity: 5.9 pg/mL. Assay Type: Sandwich. Standard: 1000 pg/mL. Sample Type: Serum, plasma, tissue homogenates and other biological fluids.. Assay duration: 3.5h
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the usability of this website, serve for direct advertising or simplify interaction with other websites and social networks, will only be used with your consent.
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