Rat Fatty-acid amide hydrolase 1 (FAAH) ELISA kit

Sample Types: serum, plasma, tissue homogenates Detection Range: 0.156 ng/mL-10 ng/mL Sensitivity: 0.039 ng/mL Assay Principle: quantitative Measurement: SandwichAssay Time: 1-5h Sample Volume: 50-100ulDetection Wavelength: 450 nmProtein function: Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z, 8Z, 11Z, 14Z-eicosatetraenoyl)- ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed:9122178, PubMed:9299394, PubMed:9790682, PubMed:10526230, PubMed:11128635, PubMed:15533037, PubMed:17649977). Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (PubMed:9122178, PubMed:11128635). It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z, 8Z, 11Z, 14Z- eicosatetraenoyl)-glycerol) (PubMed:10526230, PubMed:15533037, PubMed:17649977). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N- fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids. [The UniProt Consortium]