Rat Cox4i1 (Cytochrome c oxidase subunit 4 isoform 1, mitochondrial) ELISA Kit (RTFI01447)

Rat Cox4i1 (Cytochrome c oxidase subunit 4 isoform 1, mitochondrial) ELISA Kit from Assay Genie is a pre-coated immunoassay with a high sensitivity and broad range and has been designed to measure Rat Cox4i1 (Cytochrome c oxidase subunit 4 isoform 1, mitochondrial) in serum, plasma & cell culture supernatant samples. The Rat Cox4i1 (Cytochrome c oxidase subunit 4 isoform 1, mitochondrial) ELISA Kit accurately measures natural Rat Cox4i1 (Cytochrome c oxidase subunit 4 isoform 1, mitochondrial) levels quantified versus standard curves obtained and is based on antibodies raised against a recombinant Rat Cox4i1 (Cytochrome c oxidase subunit 4 isoform 1, mitochondrial) peptide. Protein function: Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol- cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunbit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. [The UniProt Consortium]