N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) BioAssay(TM) ELISA Kit (Mouse)

N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NT-ProBNP in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 6.9-5000pg/ml, Sensitivity: 2.5pg/ml, Intra-Assay CV: <10% , Inter-Assay CV: <12%, Kit Components: *027010A: Microtiter Strips, 1x48 wells or 1x96 wells, *027010B: Standard, 2x1 vial, 027010C: Standard Diluent, 1x10ml or 1x20ml, *027010D: Detection Reagent A (green), 1x60ul or 1x120ul, *027010E: Detection Reagent B (red), 1x60ul or 1x120ul, 027010F: Assay Diluent A, 1x6ml or 1x12ml, 027010G: Assay Diluent B, 1x6ml or 1x12ml, 027010H: TMB Substrate, 1x4.5ml or 1x9ml, *027010J: Positive Control, 215.6ng/ml, 1x1 vial, 027010K: Stop Solution, 1x3ml or 1x6ml, 027010L: Wash Buffer, 30X, 1x10ml or 1x20ml, Precaution: The Stop Solution (027010K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *027010A, *027010B, *027010D, *027010E and *027010J at -20°C. After reconstitution of *027010J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.