KLK1 BioAssay(TM) ELISA Kit, Human

Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Intended Use: KLK1 BioAssay(TM) ELISA Kit is a sandwich ELISA for quantitative detection of human KLK1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Range: 156pg/ml-10,000pg/ml, Sensitivity: <10 pg/ml, Specificity: Natural and recombinant human KLK1, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Kit Components: 143943A: Microtiter Plate, 1x96 wells, *143943B: KLK1 Standard, 2x1 vial, 143943C: Anti-human KLK1 (Biotin), 1x130ul (1:100 dilution), 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample Diluent Buffer, 1x30ml, 143698: Antibody Diluent Buffer, 1x12ml, 143699: ABC Diluent Buffer, 1x12ml, 143700: TMB Color Developing Agent, 1x10ml, 143701: TMB Stop Solution, 2N H2SO4, 1x10ml, Storage and Stability: Store *143943B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. , 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS or PBS., 3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS or PBS., 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 25-30 minutes., 5. Add TMB stop solution and read.