Interleukin 31 (IL31) BioAssay(TM) ELISA Kit (Mouse)

Interleukin 31 (IL31) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL31 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 7.8-500pg/ml, Sensitivity: 3.1pg/ml, Intra-Assay CV: <10% , Inter-Assay CV: <12%, Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately. Kit Components: *026218A: Microtiter Strips, 1x96wells, Pre-coated, ready to use, *026218B: Standard, 2x1vial, 026218C: Standard Diluent, 1x20ml, *026218D: Detection Reagent A, 1x120ul, *026218E: Detection Reagent B, 1x120ul, 026218F: Assay Diluent A, 1x12ml, 026218G: Assay Diluent B, 1x12ml, 026218H: TMB Substrate, 1x9ml, *026218J: Positive Control, 48.05pg/ml, 1x1vial, 026218K: Stop Solution, 1x6ml, 026218L: Wash Buffer, 30X, 1x20ml, Precaution: The Stop Solution (026218K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *026218A, *026218B, *026218D, *026218E and *026218J at -20°C. After reconstitution of *026218J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.